Victoria Police evacuated Melbourne LGBTI radio station JOY 94.9 on Tuesday night after the station was sent an email threat, which is now under investigation.
The threat comes as the marriage equality plebiscite debate continues to dominate public discourse.
Police arrived at the radio station at about 8.30pm on Tuesday to evacuate the building and perform a security check.
JOY 94.9 CEO Tennille Moisel told The Huffington Post Australia the threat was quite specific which Victoria Police are investigating.
"The fact that we have received such a threat is extremely unusual," Moisel said.
"We are very concerned about the increasing vitriol directed against our community through the marriage plebiscite debate, and the increasing risk it poses for our organisation and many others.
"It also correlates with increasing requests for support that we've been receiving along with other LGBTI support organisations."
JOY 94.9 provides a voice for those in LGBTI community and has broadcast arguments against the marriage equality plebiscite.
In a statement released on Wednesday, JOY 94.9 President Jed Gilbert said the radio station would continue debating marriage equality and the plebiscite on air.
"This threat last night highlights the damage that can be done through a divisive public debate about our community's aspiration to be equal members of society," Gilbert said in a statement.
"After the Orlando tragedy JOY reviewed its security arrangements, and these were executed in response to the threat last night."
The radio station's security will now remain on a heightened alert.
Prime Minister Malcolm Turnbull introduced a marriage equality plebiscite bill to Parliament last week, proposing February 11 as the date the nation would vote.
The bill -- which is yet to pass -- has faced harsh criticisms from Labor, The Greens and some crossbenchers who claim the issue being thrown into the public debate could provide a platform for detrimental hate speech and further divide.
The Huffington Post Australia has contacted JOY 94.9 for further comment.